Dissociation and Rate Constants of Some Human Liver Alcohol Dehydrogenase Isoenzymes.

Alcohol dehydrogenase isoenzymes nomenclature.

We read with a great pleasure the article ‘Rare ADH variant constellations are specific for alcohol dependence’ by Zuo et al. (2012). We want to direct the speciality about the alcohol dehydrogenase (ADH) isoenzyme nomenclature to the readers of Alcohol and Alcoholism. Namely, the authors use an old isoenzyme nomenclature what can cause some confusion to not enough familiar readers. Electrophor...

Interconversion of Isoenzymes of Drosophila Alcohol Dehydrogenase

Drosophila alcohol dehydrogenase and its subunits were examined for their physical characteristics. The intact enzyme was shown to have a molecular weight of approximately 60,000. The smallest apparent subunit was obtained by dissociating the enzyme with sodium dodecylsulfate and the molecular weight obtained was 7,500. Evidence for three partially dissociated forms was obtained; the subunits o...

First Principles Determination of Dissociation Rate Constants

Evaluation of rate constants for enzyme-catalysed reactions by the jackknife technique. Application to liver alcohol dehydrogenase.

Steady-state measurements of enzyme-catalysed reactions are capable of providing more information about the rate constants of the individual steps than is commonly obtained. We have applied a combination of the jackknife and non-linear regression techniques to measurements of the rate of oxidation of ethanol by NAD+, catalysed by alcohol dehydrogenase from horse liver. This has permitted values...

Lactate dehydrogenase isoenzymes of human semen.

1. A lactate dehydrogenase isoenzyme present in human spermatozoa and semen was isolated and characterized biochemically in term of its pH for optimum activity and by means of K(m) values for lactate, NAD(+) and NAD analogues. The results were compared with those obtained with the human heart-type and the liver-type lactate dehydrogenase isoenzymes. 2. The enzyme was characterized by its resist...